This paper proposes a GeneraLIst encoder-Decoder (GLID) pre-training method for better handling various downstream computer vision tasks. While self-supervised pre-training approaches, e.g., Masked Autoencoder, have shown success in transfer learning, task-specific sub-architectures are still required to be appended for different downstream tasks, which cannot enjoy the benefits of large-scale pre-training. GLID overcomes this challenge by allowing the pre-trained generalist encoder-decoder to be fine-tuned on various vision tasks with minimal task-specific architecture modifications. In the GLID training scheme, pre-training pretext task and other downstream tasks are modeled as "query-to-answer" problems, including the pre-training pretext task and other downstream tasks. We pre-train a task-agnostic encoder-decoder with query-mask pairs. During fine-tuning, GLID maintains the pre-trained encoder-decoder and queries, only replacing the topmost linear transformation layer with task-specific linear heads. This minimizes the pretrain-finetune architecture inconsistency and enables the pre-trained model to better adapt to downstream tasks. GLID achieves competitive performance on various vision tasks, including object detection, image segmentation, pose estimation, and depth estimation, outperforming or matching specialist models such as Mask2Former, DETR, ViTPose, and BinsFormer.