We present Iterative Vision-and-Language Navigation (IVLN), a paradigm for evaluating language-guided agents navigating in a persistent environment over time. Existing Vision-and-Language Navigation (VLN) benchmarks erase the agent’s memory at the beginning of every episode, testing the ability to perform cold-start navigation with no prior information. However, deployed robots occupy the same environment for long periods of time. The IVLN paradigm addresses this disparity by training and evaluating VLN agents that maintain memory across tours of scenes that consist of up to 100 ordered instruction-following Room-to-Room (R2R) episodes, each defined by an individual language instruction and a target path. We present discrete and continuous Iterative Room-to-Room (IR2R) benchmarks comprising about 400 tours each in 80 indoor scenes. We find that extending the implicit memory of high-performing transformer VLN agents is not sufficient for IVLN, but agents that build maps can benefit from environment persistence, motivating a renewed focus on map-building agents in VLN.